Fertilization Embryo Culture The frequently asked question is the difference between the IVF (in vitro fertilization) and the microinjection (ICSI/ intracytoplasmic sperm injection). In classical method (IVF), 50-100.000 sperms are placed around each egg to allow fertilization. With microinjection method, a selected sperm is directly injected into the egg prepared by some operations. After egg collection, the eggs…