Fertilization Embryo Culture The frequently asked question is the difference between the IVF (in vitro fertilization) and the microinjection (ICSI/ intracytoplasmic sperm injection). In classical method (IVF), 50-100.000 sperms are placed around each egg to allow fertilization. With microinjection method, a selected sperm is directly injected into the egg prepared by some operations. After egg collection, the eggs…
Egg Collection in IVF Treatment What is Egg Collection (OPU)? Egg collection is a simple surgical procedure performed with vaginal aspiration in accompany of ultrasound. Egg pick-up process is performed under general anesthesia (sedation anesthesia) at our center. The vagina is entered by ultrasound probe to identify mature follicles then the follicle is entered by a needle. The eggs are…
The eggs collected in the OPU process are surrounded by thousands of cumulus cells, defined as COC (Cumulus oocyte complex), which support the development and viability of the egg during its developmental stage and until fertilization. If microinjection (ICSI) is planned, the cumulus cells are separated from the egg by a process defined as denudation in order to identify mature…
The sperm of the man is combined with mature eggs. Sperm and eggs can be placed side by side and natural fertilization can be expected. If fertilization is unlikely, the medical team may decide to inject sperm into the egg. This process is known as intracytoplasmic sperm injection or ICSI. This method is used when it is thought that it may increase the chance…
This process, also called Assisted Nesting; is the application of the laser to help the embryo to separate itself from the enveloping membrane (zona pellucida). Assisted Hatching (AHA) technique helps the embryo to hold onto the uterus. Assisted Hatching (AHA) can be applied in recurrent IVF failures. It is thought that this method can improve the results in cases where…
For each patient undergoing IVF treatment, embryo transfer is the most stressful and exciting process of treatment. After weeks of drug use and follow-up, eggs were collected and the embryo development was followed up enthusiastically. It’s the last step. Insertion of the embryo into the uterus is the last procedure before the patient is formally pregnant. Embryo transfer is a simple…
Embryo cryopreservation is the process of freezing and storing embryos and is a part of most in vitro fertilization programs. The process is chosen for reasons as varied as providing an additional chance for pregnancy, or saving embryos in the face of certain medical treatments. What is in vitro fertilization (IVF)? Many women and their partners try for a long…